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Posted: September 28th, 2022

Flow Cytometry-Based Functional Assays

Flow Cytometry-Based Functional Assays
Flow-cytometry-based functional assays are used to elucidate the drug mechanism. The ex vivo stimulation of drug cells, cytokines, and antibodies is part of the ex vivo drugs. The markers staining follows the ex vivo drugs to stage the amount of biological response produced. Several operational parameters can be measured by Flow cytometry (Alrubayyi, Schuetz, Jongrakthaitae, Paolino, Ake & Eller, 2018). It shows how biologists and clinicians are interested in the study of cells. How the cells behave in their functionality is the only thing that distinguishes the assays. The phenotype doesn’t matter a lot.
The multiplex essay uses beads that are magnetic, in just one experiment, to continuously measure the number of analytes. The bone marrow immunological analysis does not allow accurate identification of the morphology in the blast cells. CD34 immunotyping of blasts that exist in the healthy bone marrow differs from when there is an increase in the bone marrow blasts (Oertel & Huhn, 1994). A decrease means that the CD34 type is negative. The purpose of the assay is to determine the amount of substance in the human body. Mostly it is used to measure the number of thyroid hormones in the blood.
Generally, Flow-cytometry-based functional assays are used to elucidate the drug mechanism. The functional behavior of the cells is the only feature that distinguishes the assays. The multiplex essay uses beads that are magnetic, in just one experiment, to continuously measure the number of analytes. CD34 immunotyping of blasts that exist in the normal bone marrow differs from when there is an increase in the bone marrow blasts. The purpose of the assay is to determine the amount of substance in the human body.

References
Alrubayyi, A., Schuetz, A., Lal, K. G., Jongrakthaitae, S., Paolino, K. M., Ake, J., A., … & Eller, M. A. (2018). Flow cytometry-based assay that simultaneously measures cytotoxicity and monocyte mediated antibody-dependent effector activity. Journal of immunological methods, 462, 74-82.
Oertel, J., & Huhn, D. (1994). CD34 immunophenotyping of blasts in myelodysplasia. Leukemia & lymphoma, 15(1-2), 65-69.

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